Adult Brain Dissociation Kit 130-107-677 from Miltenyi Biotec

(1) Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophagesSTAR ProtocolsDecember 1, 2023Ignazio Antignano, Lily Keane, Melania CapassoMiltenyi Biotec (https://www.miltenyibiotec.com/DE-en/products/adult-brain-dissociation-kit-mouse-and-rat.html#130-107-677). Troubleshooting 4 and troubleshooting 5. Ensure to remove debris using the debris removal step according

(2) RIPK3 promotes brain region-specific interferon signaling and restriction of tick-borne flavivirus infectionPLOS PathogensNovember 27, 2023Marissa Lindman, Juan P. Angel, Irving Estevez, Nydia P. Chang, Tsui-Wen Chou, Micheal McCourt, Colm Atkins, Brian P. Danielsminor modifications. Adult cerebella were dissociated using an Adult Brain Dissociation Kit (Miltenyi, #130-107-677). Cells were layered over a step gradient of 60% and 35% isotonic Percoll (Cytiva, #17-0891-02) and

(3) Single-cell transcriptomics reveals the brain evolution of web-building spidersNature Ecology & EvolutionNovember 2, 2023Pengyu Jin, Bingyue Zhu, Yinjun Jia, Yiming Zhang, Wei Wang, Yunxiao Shen, Yu Zhong, Yami Zheng, Yang Wang, Yan Tong, et al.tissue was dissociated into single cells using the Adult Brain Dissociation kit (Miltenyi Biotec number 130-107-677) with these modifications: (1) after termination of the gentleMACS programme, the C-tube with the sample

(4) Iron overload induces cerebral endothelial senescence in aged mice and in primary culture in a sex-dependent mannerAging cellNovember 1, 2023Noh B, Blasco-Conesa MP, Rahman SM, Monga S, Ritzel R, Guzman G, Lai YJ, Ganesh BP, Urayama A, McCullough LD, et al.m/o old C57BL/6J male and female mice using the Adult Brain Dissociation Kit (MACS Miltenyi Biotec, #130‐107‐677) as previously described (Noh et al., 2022). Briefly, brain tissue was mechanically and chemically homogenized,

(5) Single-cell analysis of 5-aminolevulinic acid intraoperative labeling specificity for glioblastomaJournal of NeurosurgerySeptember 22, 2023Zhouzerui Liu, Angeliki Mela, Michael G. Argenziano, Matei A. Banu, Julia Furnari, Corina Kotidis, Colin P. Sperring, Nelson Humala, Aayushi Mahajan, Jeffrey N. Bruce, et al.tissue samples were dissociated using the adult brain dissociation kit (Miltenyi Biotec, catalog no. 130-107-677) on a gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s instructions,

(6) DNA hypomethylation promotes the expression of CASPASE-4 which exacerbates neuroinflammation and amyloid-β deposition in Alzheimer's disease The Ohio State University College of MedicinebioRxiv : the preprint server for biologySeptember 1, 2023Daily KP, Badr A, Eltobgy M, Estfanous S, Whitham O, Tan MH, Carafice C, Krause K, McNamara A, Hamilton K, et al.of CASP11 by immunoblot. Microglia were isolated by MACS neural dissociation kit (Miltenyi Biotec, 130-107-677) followed by CD11b magnetic bead (Miltenyi Biotec, 130-093-634) isolation technique to positively select

(7) Accelerated aging in mice with astrocytic redox imbalance as a consequence of SOD2 deletionAging CellSeptember 1, 2023Konstantinos Tsesmelis, Gandhari Maity‐Kumar, Dana Croner, Jasmin Sprissler, Miltiadis Tsesmelis, Tabea Hein, Bernd Baumann, Thomas Wirthbrain Mouse adult brain tissue was dissociated with an adult mouse brain dissociation kit (Miltenyi Biotec, #130‐107‐677). Dissociated cells, after removal of debris and red blood cells, were separated magnetically with an

(8) The Role of PRRC2B in Cerebral Vascular Remodeling Under Acute Hypoxia in MiceAdvanced ScienceSeptember 1, 2023Shuoshuo Li, Wenyu Hu, Shenghui Gong, Ping Zhang, Jinbo Cheng, Shukun Wang, Yingyi Wang, Wenjun Shi, Qianqian Li, Fengchao Wang, et al.endothelial cells separated from hypoxia‐and‐hypobaric‐exposure mice and control‐group mice (Miltenyi Biotec, 130107677). The brain parenchyma was harvested after mice were perfused with saline. The brain parenchyma was

(9) Rescue of Alzheimer's disease phenotype in a mouse model by transplantation of wild-type hematopoietic stem and progenitor cellsCell reportsAugust 29, 2023Mishra P, Silva A, Sharma J, Nguyen J, Pizzo DP, Hinz D, Sahoo D, Cherqui S.single-cell suspensions according to manufacture instruction using the adult brain dissociation kit (130-107-677, Miltenyi Biotec, Bergisch-Gladbach, Germany). For flow cytometry analysis, single cell suspensions

(10) Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouseActa neuropathologica communicationsAugust 21, 2023Crivelli SM, Quadri Z, Vekaria HJ, Zhu Z, Tripathi P, Elsherbini A, Zhang L, Sullivan PG, Bieberich E.microglia were isolated and cultivated from adult WT at 4 weeks of age using MACS® Tissue Dissociation Kit (130-107-677) from Miltenyi Biotec in 12 well plates. Microglia were cultured in DMEM (Corning, 10-013-CV) containing

(11) Brain cell type specific proteomics approach to discover pathological mechanisms in the childhood CNS disorder mucolipidosis type IVFrontiers in Molecular NeuroscienceAugust 7, 2023Madison Sangster, Sanjid Shahriar, Zachary Niziolek, Maria Carla Carisi, Michael Lewandowski, Bogdan Budnik, Yulia Grishchuktrehalose). After a short spin-down and removal of supernatant, the Adult Brain Dissociation kit (Miltenyi #130–107-677, RRID:SCR_020295) was used with a few modifications to obtain a single cell solution. In enzyme mix

(12) microRNA-132 regulates gene expression programs involved in microglial homeostasisiScienceJune 16, 2023Hannah Walgrave, Amber Penning, Giorgia Tosoni, Sarah Snoeck, Kristofer Davie, Emma Davis, Leen Wolfs, Annerieke Sierksma, Mayte Mars, Taofeng Bu, et al.Hippocampi were dissociated enzymatically using the Adult Brain Dissociation Kit, mouse and rat (Cat#130-107-677, Miltenyi Biotec) for 15 min at 37°C, with 5 μM Actinomycin D added. Subsequently mechanical dissociation

(13) Topical and systemic GLP-1R agonist administration both rescue retinal ganglion cells in hypertensive glaucomaFrontiers in Cellular NeuroscienceJune 9, 2023Emily C. N. Lawrence, Michelle Guo, Turner D. Schwartz, Jie Wu, Jingwen Lu, Sergei Nikonov, Jacob K. Sterling, Qi N. Cuipoor tissue processing resulting in impaired image quality. Miltenyi Adult Brain Dissociation Kit (130-107-677, Miltenyi Biotec, Bergisch Gladbach, Germany) and MACS magnetic cell separation system was used to enrich

(14) Lymphocyte-Related Immunomodulatory Therapy with Siponimod (BAF-312) Improves Outcomes in Mice with Acute Intracerebral HemorrhageAging and diseaseJune 1, 2023Zhang Z, Li Y, Shi J, Zhu L, Dai Y, Fu P, Liu S, Hong M, Zhang J, Wang J, et al.after ICH. Single cell suspensions were prepared using an adult mouse brain tissue dissociation kit (130-107-677, Miltenyi Biotec) as previously described [13, 74]. Briefly, mice (n = 6 per group) were perfused with

(15) Acute Stress, Induced by IFNγ + Aβ, and Chronic Stress, Induced by Age, Affect Microglia in a Sex-Specific MannerMolecular NeurobiologyJune 1, 2023Virginia Mela, Aline Sayd Gaban, Paul Marie Shatz, Marie-Victoire Guillot-Sestier, Marina A. Lynchgentle-MACS Dissociator (Miltenyi Biotec, UK) in combination with the AdultBrain Dissociation Kit (130–107-677; Miltenyi Biotec UK); filtered through a MACS SmartStrainer (70 µm); washed with D-PBS containing PBS,

(16) Glial progenitor heterogeneity and key regulators revealed by single-cell RNA sequencing provide insight to regeneration in spinal cord injuryCell reportsMay 30, 2023Wei H, Wu X, Withrow J, Cuevas-Diaz Duran R, Singh S, Chaboub LS, Rakshit J, Mejia J, Rolfe A, Herrera JJ, et al.tissue dissociation was performed by using adult brain tissue dissociation kit (Miltenyi Biotec; cat #130-107-677) as per manufactures instruction. Thereafter, collected cell suspensions were passed through a 70-μm

(17) Transcriptomic Analysis of mRNA Expression Profiles in the Microglia of Mouse Brains Infected with Rabies Viruses of Varying VirulenceVirusesMay 23, 2023Jundan Liu, Wangchang Li, Dongling Yu, Rong Jin, Hualin Hou, Xiaoqing Ling, Abraha Bahlbi Kiflu, Xiankai Wei, Xiaogan Yang, Xiaoning Li, et al.to disperse the cells using an adult brain dissociation kit (Miltenyi, Bergisch Gladbach, Germany, 130-107-677) according to the manufacturer’s instructions. Then, the microglia were positively selected using an

(18) Modulation of microglial metabolism facilitates regeneration in demyelinationiScienceMay 19, 2023Qin C, Yang S, Chen M, Dong MH, Zhou LQ, Chu YH, Shen ZX, Bosco DB, Wu LJ, Tian DS, et al.#40739ES50 Mitotracker,YEASEN,Cat #40740ES50 PLX5622,Research Diets,Cat #28927 Adult brain dissociation kit,Miltenyi Biotec,Cat #130-107-677 Rneasy micro kit,Qiagen,Cat #74004 L-α-Lysolecthin,Sigma,Cat #L4129 7-AAD,BD Bioscience,Cat #559925 MS

(19) Loss of Astrocytic µ Opioid Receptors Exacerbates Aversion Associated with Morphine Withdrawal in Mice: Role of Mitochondrial RespirationCellsMay 17, 2023Kateryna Murlanova, Yan Jouroukhin, Ksenia Novototskaya-Vlasova, Shovgi Huseynov, Olga Pletnikova, Michael J. Morales, Yun Guan, Atsushi Kamiya, Dwight E. Bergles, David M. Dietz, et al.of astrocytes from adult mouse brain” Miltenyi Biotec protocol for the Adult Brain Dissociation kit (130-107-677, Miltenyi Biotec, Bergisch Gladbach, Germany) and anti-ACSA-2 MicroBead kit (130-097-678, Miltenyi Biotec).

(20) Novel CH25H+ and OASL+ microglia subclusters play distinct roles in cerebral ischemic strokeJournal of NeuroinflammationMay 15, 2023Yueman Zhang, Yunlu Guo, Ruqi Li, Tingting Huang, Yan Li, Wanqin Xie, Chen Chen, Weijie Chen, Jieqing Wan, Weifeng Yu, et al.and 100μL Enzyme R and 25μL Enzyme A (Adult Brain Dissociation Kit, mouse and rat, Miltenyi Biotec, 130–107–677) for 30 min at 37 °C. After digestion, samples were sieved through a 70 µm cell strainer, and centrifuged

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6/22/2016Single Cell isolation from Adult Mouse Brain Sivaraman NatarajanWe use in our lab this Kit combined with MACS gentle dissociator to dissociate the adult brain from the mouse. The protocol is highly optimized by miltenyi Biotech for mouse brain that yileds high ... Read Review

Adult Brain Dissociation Kit, mouse and rat (for 50 preparations)

Adult Brain Dissociation Kit from Miltenyi Biotec

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Adult Brain Dissociation Kit from Miltenyi Biotec

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